Timescales of IP3-Evoked Ca2+ Spikes Emerge from Ca2+ Puffs Only at the Cellular Level
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چکیده
منابع مشابه
Timescales of IP3-Evoked Ca 2D Spikes Emerge from Ca Puffs Only at the Cellular Level
The behavior of biological systems is determined by the properties of their component molecules, but the interactions are usually too complex to understand fully how molecular behavior generates cellular behavior. Ca2þ signaling by inositol trisphosphate receptors (IP3R) offers an opportunity to understand this relationship because the cellular behavior is defined largely by Ca2þ-mediated inter...
متن کاملTimescales of IP(3)-evoked Ca(2+) spikes emerge from Ca(2+) puffs only at the cellular level.
The behavior of biological systems is determined by the properties of their component molecules, but the interactions are usually too complex to understand fully how molecular behavior generates cellular behavior. Ca(2+) signaling by inositol trisphosphate receptors (IP(3)R) offers an opportunity to understand this relationship because the cellular behavior is defined largely by Ca(2+)-mediated...
متن کاملCytosolic [Ca2+] regulation of InsP3-evoked puffs.
InsP3-mediated puffs are fundamental building blocks of cellular Ca2+ signalling, and arise through the concerted opening of clustered InsP3Rs (InsP3 receptors) co-ordinated via Ca2+-induced Ca2+ release. Although the Ca2+ dependency of InsP3Rs has been extensively studied at the single channel level, little is known as to how changes in basal cytosolic [Ca2+] would alter the dynamics of InsP3-...
متن کاملEnhanced ER Ca2+ store filling by overexpression of SERCA2b promotes IP3-evoked puffs.
Liberation of Ca(2+) from the endoplasmic reticulum (ER) through inositol trisphosphate receptors (IP(3)R) is modulated by the ER Ca(2+) content, and overexpression of SERCA2b to accelerate Ca(2+) sequestration into the ER has been shown to potentiate the frequency and amplitude of IP(3)-evoked Ca(2+) waves in Xenopus oocytes. Here, we examined the effects of SERCA overexpression on the element...
متن کاملEndogenous signalling pathways and caged IP3 evoke Ca2+ puffs at the same abundant immobile intracellular sites
The building blocks of intracellular Ca2+ signals evoked by inositol 1,4,5-trisphosphate receptors (IP3Rs) are Ca2+ puffs, transient focal increases in Ca2+ concentration that reflect the opening of small clusters of IP3Rs. We use total internal reflection fluorescence microscopy and automated analyses to detect Ca2+ puffs evoked by photolysis of caged IP3 or activation of endogenous muscarinic...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.10.030